Tris(hydroxymethyl) aminomethane
Tris(hydroxymethyl)aminoethane
CAS: 77-86-1
Molecular Formula: C4H11NO3
Tris(hydroxymethyl) aminomethane - Names and Identifiers
Tris(hydroxymethyl) aminomethane - Physico-chemical Properties
| Molecular Formula | C4H11NO3 |
| Molar Mass | 121.14 |
| Density | 1,353 g/cm3 |
| Melting Point | 167-172°C(lit.) |
| Boling Point | 219-220°C10mm Hg(lit.) |
| Flash Point | 219-220°C/10mm |
| Water Solubility | 550 g/L (25 ºC) |
| Solubility | H2O: 4M at20°C, clear, colorless |
| Vapor Presure | 0.0267 hPa (20 °C) |
| Appearance | White powder |
| Color | white |
| Maximum wavelength(λmax) | ['λ: 260 nm Amax: 0.10', , 'λ: 280 nm Amax: 0.08'] |
| Merck | 14,9772 |
| BRN | 741883 |
| pKa | 8.1(at 25℃) |
| PH | 10.5-12.0(4 m in water, 25 °C) |
| Storage Condition | 20-25°C |
| Stability | Stable. Incompatible with bases, strong oxidizing agents. Protect from moisture. |
| Sensitive | Hygroscopic |
| Refractive Index | 1.4170 (estimate) |
| MDL | MFCD00004679 |
| Physical and Chemical Properties | Melting point 167-172°C boiling point 219-220°C (10 mmHg) water-soluble 550g/L (25°C) |
| Use | Mainly used in pharmaceutical intermediates |
Tris(hydroxymethyl) aminomethane - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S37/39 - Wear suitable gloves and eye/face protection S24/25 - Avoid contact with skin and eyes. S23 - Do not breathe vapour. |
| WGK Germany | 2 |
| RTECS | TY2900000 |
| FLUKA BRAND F CODES | 3 |
| TSCA | Yes |
| HS Code | 29221980 |
| Hazard Class | IRRITANT |
| Toxicity | LD50 orally in Rabbit: > 5000 mg/kg LD50 dermal Rat > 5000 mg/kg |
Tris(hydroxymethyl) aminomethane - Upstream Downstream Industry
| Raw Materials | Paraformaldehyde Paraformaldehyde Methyl alcohol Methyl alcohol tris(hydroxymethyl)nitromethane Nitromethane |
Tris(hydroxymethyl) aminomethane - Standard
Authoritative Data Verified Data
This product is 2-amino -2-hydroxymethyl -1, 3-propanediol. Calculated as the dried product, the content of C4H · N 0 3F should be less than 99.0%.
Last Update：2024-01-02 23:10:35
Tris(hydroxymethyl) aminomethane - Trait
Authoritative Data Verified Data
- This product is white crystal.
- This product is soluble in water and soluble in ethanol.
melting point
The melting point of this product (General rule 0612 second method) is 168 ~ 172C.
Last Update：2022-01-01 11:24:32
Tris(hydroxymethyl) aminomethane - Differential diagnosis
Authoritative Data Verified Data
- take 0.2g of this product, add water lm l to dissolve, add salicylaldehyde saturated solution lm l and 2 drops of glacial acetic acid, that is yellow.
- the position and color of the main spot displayed by the test solution (2) under the item of related substances should be the same as that of the main spot of the reference solution.
- The infrared absorption spectrum of this product should be consistent with that of the control (Spectrum set 408).
Last Update：2022-01-01 11:24:33
Tris(hydroxymethyl) aminomethane - Exam
Authoritative Data Verified Data
alkalinity
take this product l .O g, add water dissolved in 20ml, according to the law (General 0631), p H value should be 10.0~11. 5.
clarity and color of solution
Take 2. 5g of this product, add 50ml of New boiling and cooling water to dissolve, check according to law (General rule 0901 and general rule 0902), the solution should be clear, almost colorless. If it is turbid, it should not be more concentrated than No. 1 turbidity standard solution (General rule 0902).
hydride
Take 10ml of solution under the items of clarity and color of solution, check according to law (General rule 0801), and standard gasified sodium solution 5.0ml of the control solution should not be more concentrated (0.01%).
Related substances
take 0.20g of this product, put it in a 10ml measuring flask, dissolve it with water lm l, dilute it to the scale with methanol, shake it well, and use it as a test solution (1 ), take lm l accurately, put it in a 10ml measuring flask, dilute it to the scale with methanol, shake it well, and use it as a test solution (2). Another 20mg of tromethamine control was added into a 10ml measuring flask, dissolved in methanol and diluted to the scale, and then shaken to be used as a control solution. The sample solution (l) was accurately measured and placed in a 100ml measuring flask. The solution was diluted to the scale with methanol and shaken to obtain a control solution. According to the thin layer chromatography (General 0502) test, draw 10M1 of each of the above 4 solutions, respectively on the same silica gel G thin layer plate (such as Merck thin layer plate or equivalent thin layer plate) pre-developed in methanol, with ammonia-isopropyl alcohol (1: 9) as the developing agent, in
1 05T: After drying, spray with potassium permanganate color solution (take potassium permanganate 0.5g, add 1 0g /L sodium carbonate solution 100ml to dissolve) color, place about 10 minutes after testing, test Solution (1) such as impurity spots, compared with the control solution showed the main spot, not deeper.
loss on drying
take this product and dry it to constant weight under reduced pressure at 80°C. The weight loss shall not exceed 0 .6% (General rule 0831).
ignition residue
The l .O g of this product shall be taken for inspection according to law (General rule 0841), and the remaining residue shall not exceed.
Iron Salt
take this product l .O g, put in 10ml measuring flask, add water to dissolve and dilute to the scale, check according to law (General Rule 0 8 0 7 ) , and standard iron solution 1. Compared with the control solution made of 0M l, it should not be deeper (0.001%).
nickel salt
take this product l .O g, after dissolving with 10ml of water, add 2ml of ammonia test solution lm l and dimethylglyoxime test solution, and place it for 10 minutes. If it is colored, it should be mixed with standard nickel solution (nickel ammonium sulfate 0 .6 7 3 0G, put in 1000ml measuring flask, add appropriate amount of water to dissolve and dilute to the scale, shake. Take 10m l for precision measurement, put it in a measuring flask, dilute it to scale with water, and shake well) 1. 5m l compared with the control solution made by the same method, no deeper (0.0015%).
Heavy metals
The residue left under the item of taking the ignition residue shall not contain more than 10 parts per million of heavy metal when examined by law (General Principles 0821, Law II).
endotoxin (for injection)
take this product and check it according to law (General rule 1 1 4 3). The amount of endotoxin in each lm g tromethamine should be less than 0.03EU.
sterile (preparation suitable for non-terminal sterilization process or non-sterilization process)
take this product, according to law inspection (General rule 1 1 0 1 ) , should comply with the provisions.
Last Update：2022-01-01 11:24:34
Tris(hydroxymethyl) aminomethane - Content determination
Authoritative Data Verified Data
take this product about 0 .2 5G, precision weighing, add water 80ml to dissolve, add methyl red indicator solution 2~3 drops, with hydrochloric acid titration solution (0. lmol/L) titration, that is. Δlm l hydrochloric acid titrant (0. lm o l/L) corresponds to 12.11mg mc4h11n03.
Last Update：2022-01-01 11:24:34
Tris(hydroxymethyl) aminomethane - Category
Authoritative Data Verified Data
pharmaceutical excipients, acid-base balance regulator.
Last Update：2022-01-01 11:24:35
Tris(hydroxymethyl) aminomethane - Storage
Authoritative Data Verified Data
light shielding, sealed storage.
Last Update：2022-01-01 11:24:35
Tris(hydroxymethyl) aminomethane - Reference Information
| pH range of acid-base indicator discoloration | 7 - 9 |
| NIST chemical information | information provided by: webbook.nist.gov (external link) |
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Overview | Tris Chinese name: Tris; Tromethamine; Tromethamine; 2-amino -2-(hydroxymethyl)-1, 3-propanediol. Is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate, benzene, insoluble in ether, carbon tetrachloride, copper, aluminum corrosion, irritant chemicals. |
| indication | tromethamine is a sodium-free amino buffer base that reacts with H2CO3 in body fluid to reduce H2CO3, at the same time generate HCO32-, can uptake hydrogen ions and correct acidemia, its role is strong, and can penetrate the cell membrane, commonly used in acute metabolic and respiratory acidemia. |
| buffer properties | Tris is a weak base with a pKa of 8.1 at 25 °c, the effective buffering range of the Tris buffer was between pH 9.2 and. The pH of the aqueous solution of Tris base is about 10.5, and hydrochloric acid is generally added to adjust the pH value to the desired value to obtain a buffer of the pH value. However, attention should be paid to the effect of temperature on pKa of Tris. |
| synthesis method | method one Firstly, The nitromethane and polyoxymethylene were prepared according to a certain molar ratio of 1: 3.3, weigh 93g of paraformaldehyde into 500ml Four-necked flask, add 85g of methanol solvent and 0.3g Koh catalyst, heat and stir until completely dissolved, slowly add 55g of weighed nitromethane Dropwise for condensation reaction, the reaction temperature was maintained at 40 to 55 ° C. For 3 to 4 hours after completion of the addition (the temperature was maintained at about 55 ° C.). At the end of the reaction, one pot of condensate was put into 1L autoclave, 350g of methanol and 20g of nickel catalyst were added, the autoclave was checked for normal condition, hydrogen gas in the autoclave was replaced, and the power supply was turned on to start stirring, hydrogen gas is added for reduction reaction, the pressure is maintained at 2 ~ 3Mpa, the reaction temperature is controlled at 40~50 ℃, and the hydrogenation is completed for 2~3 hours (keeping the temperature at about 50 ℃), at the end of this period, the temperature was increased to 60 ℃ and the mixture was allowed to stand still for 1 hour, The conversion rate was 98%, the material was discharged into a ml Four-necked flask, 5g of activated carbon was added, and the mixture was stirred for 1 hour (maintained at about 50 ° C.) and filtered with a Buchner funnel, and 70g of the crude product was cooled and crystallized. Take the crude product 70G into a 500ml four-mouth flask, add 65g of deionized water and 5g of activated carbon, heat and stir, heat to 80 ℃ for 1 hour, filter with a Buchner funnel, cool and discharge, 65g of finished product was obtained by drying, the content of the finished product was 99.94%, and the absorbance (260nm) was 0.043. The methanol content of the solution was 99%. The specific steps of Method 2 are as follows:(1) 800g of industrial trimethylolmethane is added into 500ml of methanol aqueous solution, and heated to 60 ° C. For stirring and dissolving, wherein the methanol aqueous solution is purified water and methanol according to the volume ratio of 2: (2) adding 8g of charcoal activated carbon to the solution, keeping the temperature at 50 ° C. For 30 minutes, filtering while hot, and collecting the filtrate; (3) the filtrate is concentrated under reduced pressure, and the concentration temperature is 80 ° C. Until crystallization occurs, it is left to stand, and the recovered methanol can be recycled;(4) after the crystallization is separated by Suction filtration method, the product was rinsed twice with anhydrous ethanol and dried at 50 ° C. For 4 hours to obtain 680g of the finished product with a purity of 100.03%. |
| preparation of Tris buffer | 64% required pH value (25 ℃) 0.1mol/L HCI volume 7.10 45.7 7.20 44.7 7.30 43.4 7.40 42.0 7.50 40.3 7.60 38.5 7.70 36.6 7.80 34.5 7.90 32.0 8.00 29.2 8.10 26.2 8.20 22.9 8.30 19.9 8.40 17.2 8.50 14.7 8.60 12.4 8.70 10.3 8.80 8.5 8.90 7.0 |
| Application | Tris is widely used in acute metabolic and respiratory acidemia, is an alkaline buffer, it has a good buffering effect on metabolic acidosis and enzyme activity. Tris is commonly used as a biological buffer, often prepared to pH 6.8,7.4,8.0,8.8. Its structural formula and pH value vary greatly with temperature. Generally, the pH decreases by 0.03 for each degree of temperature increase. Tris is widely used in the preparation of buffers in biochemistry and molecular biology experiments. For example, TAE and TBE buffers commonly used in biochemistry experiments (for nucleic acid dissolution) require Tris; since it contains amino groups, condensation reactions with aldehydes can occur. The pH of the aqueous solution of Tris base is about 10.5, and hydrochloric acid is generally added to adjust the pH value to the desired value to obtain a buffer of the pH value. However, attention should be paid to the effect of temperature on pKa of Tris. Since the Tris buffer is a weakly alkaline solution, DNA is deprotonated in such a solution, thereby increasing its solubility. EDTA is often added to Tris-HCL buffer to make "TE buffer", which is used for DNA stabilization and storage. "TAE buffer" (Tris/Acetate/EDTA) is obtained if the pH-adjusted acid solution is changed to acetic acid, and "TBE buffer" (Tris/Borate/EDTA) is obtained if it is changed to boric acid.. These two buffers are commonly used in nucleic acid electrophoresis experiments. TAE,TBE, etc., prepared by Tris are the most commonly used reagents for DNA electrophoresis, TE (PH 8.0) is mainly used for dissolving DNA(TE is Tris plus EDTA combined). Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses; It is used for protein crystal growth under different pH conditions. The low ionic strength characteristic of Tris buffer can be used for the formation of intermediate fibers of lamin (lamin) of C. elegans, and Tris is also one of the main components of protein electrophoresis buffer. In addition, Tris is also an intermediate in the preparation of surfactants, vulcanization accelerators and some drugs, which are also commonly used as titration standards. |
| preparation method | method one kind of preparation method of Tris, the specific preparation steps are as follows:(1) trimethylolmethane is added to the methanol aqueous solution, heated to 50-70 ° C. And stirred to dissolve, wherein the mass volume ratio of the trimethylolmethane to the methanol aqueous solution is 8:3-7 in terms of g/ml, the methanol aqueous solution is prepared by mixing purified water and methanol in a volume ratio of 2:3;(2) charcoal activated carbon is added to the solution, the weight ratio of the charcoal activated carbon to trimethylolmethane is 0.5-2:100, and the filtrate is collected by hot filtration after being kept at 45-55 ° C. For 20-40 minutes;(3) the filtrate is concentrated under reduced pressure, the concentration temperature is 70-80 ° C., until the crystallization occurs, let it stand;(4) the crystallization is separated by Suction filtration, rinsed with anhydrous ethanol, and dried at 40-60 ° C. For 3-5 hours. The preparation method of the above-mentioned Tris (hydroxymethyl) aminomethane has high purity, meets the requirement of the standard reagent for the purity of Tris (hydroxymethyl) aminomethane, and the process is stable and reasonable, especially suitable for the production of KG-grade batch products, the process is simple and reasonable, the qualified rate of products is high, which is suitable for the needs of large-scale industrial production. Method di-Tris can be generated by a two-step reaction: the intermediate trimethylolnitromethane ((HOCH2)3CNO2) is first generated from nitromethane and then reduced from the intermediate to obtain Tris. |
| uses | Tris is mainly used in pharmaceutical intermediates; It can be used as a biological buffer and acid titration method standard substance; also used as biochemical reagents, fosfomycin intermediates; It can also be used as vulcanization accelerator, cosmetics (cream, lotion), mineral oil, paraffin emulsifier, biological buffer. |
| production method | the corresponding nitro compound reduction or catalytic hydrogenation method. |
| toxic substance data | information provided by: pubchem.ncbi.nlm.nih.gov (external link) |
Last Update：2024-04-10 22:29:15
Supplier List
Featured ProductsMultiple SpecificationsSpot supply
Product Name: Tris(hydroxymethyl)methyl aminomethane/THAM Request for quotationCAS: 77-86-1
Tel: +86 19849939632
Email: 2470479589@qq.com
Mobile: +86 19849939632
QQ: 2470479589
Wechat: +86 19849939632
Featured ProductsMultiple SpecificationsSpot supply
Product Name: Tris(hydroxymethyl)aminoethane Request for quotationCAS: 77-86-1
Tel: 86+027-83389957
Email: 1024042217@qq.com
Mobile: 86+18627766980
QQ: 1024042217
Wechat: 18627766980
Featured ProductsMultiple SpecificationsSpot supply
Product Name: TRIS；Tris-base；THAM Visit Supplier Webpage Request for quotationCAS: 77-86-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
Spot supply
Product Name: Tris(hydroxymethyl)aminoethane Request for quotationCAS: 77-86-1
Tel: +86-17551318830
Email: r@reformchem.com
Mobile: +86-17551318830
QQ: 3787852685
Spot supply
Product Name: Tris(hydroxymethyl)aminoethane Request for quotationCAS: 77-86-1
Tel: +86-17551318830
Email: r@reformchem.com
Mobile: +86-17551318830
QQ: 3785839865
Product Name: Tris(hydroxymethyl)aminoethane Request for quotation
CAS: 77-86-1
Tel: 17505222756
Email: pules.cn@gmail.com
Mobile: +86-17551318830
Wechat: 17505222756
CAS: 77-86-1
Tel: 17505222756
Email: pules.cn@gmail.com
Mobile: +86-17551318830
Wechat: 17505222756
Multiple SpecificationsSpot supply
Product Name: Tris Base Request for quotationCAS: 77-86-1
Tel: 15335994747
Email: 3521670276@qq.com
Mobile: 15335994747
QQ: 3521670276
Wechat: A2020071098
Product Name: Tris(hydroxymethyl)aminoethane Request for quotation
CAS: 77-86-1
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 0086 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
CAS: 77-86-1
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 0086 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
Product Name: Tris Base Request for quotation
CAS: 77-86-1
Tel: 86-10-10086 81625225
Email: yeqim@boyechemicals.com
Mobile: 86-15387090420
QQ: 1377812380
Wechat: 15387090420
CAS: 77-86-1
Tel: 86-10-10086 81625225
Email: yeqim@boyechemicals.com
Mobile: 86-15387090420
QQ: 1377812380
Wechat: 15387090420
Product Name: Tris(hydroxymethyl)aminomethane Request for quotation
CAS: 77-86-1
Tel: +86-21-56795779
Email: charles7788@worldyachem.com
Mobile: +86-13651600618
QQ: 2850607228
Wechat: 13651600618
WhatsApp: +8613651600618
CAS: 77-86-1
Tel: +86-21-56795779
Email: charles7788@worldyachem.com
Mobile: +86-13651600618
QQ: 2850607228
Wechat: 13651600618
WhatsApp: +8613651600618
Featured ProductsMultiple SpecificationsSpot supply
Product Name: Tris(hydroxymethyl)methyl aminomethane/THAM Request for quotationCAS: 77-86-1
Tel: +86 19849939632
Email: 2470479589@qq.com
Mobile: +86 19849939632
QQ: 2470479589
Wechat: +86 19849939632
Featured ProductsMultiple SpecificationsSpot supply
Product Name: Tris(hydroxymethyl)aminoethane Request for quotationCAS: 77-86-1
Tel: 86+027-83389957
Email: 1024042217@qq.com
Mobile: 86+18627766980
QQ: 1024042217
Wechat: 18627766980
Featured ProductsMultiple SpecificationsSpot supply
Product Name: TRIS；Tris-base；THAM Visit Supplier Webpage Request for quotationCAS: 77-86-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
Spot supply
Product Name: Tris(hydroxymethyl)aminoethane Request for quotationCAS: 77-86-1
Tel: +86-17551318830
Email: r@reformchem.com
Mobile: +86-17551318830
QQ: 3787852685
Spot supply
Product Name: Tris(hydroxymethyl)aminoethane Request for quotationCAS: 77-86-1
Tel: +86-17551318830
Email: r@reformchem.com
Mobile: +86-17551318830
QQ: 3785839865
Product Name: Tris(hydroxymethyl)aminoethane Request for quotation
CAS: 77-86-1
Tel: 17505222756
Email: pules.cn@gmail.com
Mobile: +86-17551318830
Wechat: 17505222756
CAS: 77-86-1
Tel: 17505222756
Email: pules.cn@gmail.com
Mobile: +86-17551318830
Wechat: 17505222756
Multiple SpecificationsSpot supply
Product Name: Tris Base Request for quotationCAS: 77-86-1
Tel: 15335994747
Email: 3521670276@qq.com
Mobile: 15335994747
QQ: 3521670276
Wechat: A2020071098
Product Name: Tris(hydroxymethyl)aminoethane Request for quotation
CAS: 77-86-1
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 0086 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
CAS: 77-86-1
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 0086 189 4982 3763
WhatsApp: 0086 189 4982 3763
Product List: View Catalog
Product Name: Tris Base Request for quotation
CAS: 77-86-1
Tel: 86-10-10086 81625225
Email: yeqim@boyechemicals.com
Mobile: 86-15387090420
QQ: 1377812380
Wechat: 15387090420
CAS: 77-86-1
Tel: 86-10-10086 81625225
Email: yeqim@boyechemicals.com
Mobile: 86-15387090420
QQ: 1377812380
Wechat: 15387090420
Product Name: Tris(hydroxymethyl)aminomethane Request for quotation
CAS: 77-86-1
Tel: +86-21-56795779
Email: charles7788@worldyachem.com
Mobile: +86-13651600618
QQ: 2850607228
Wechat: 13651600618
WhatsApp: +8613651600618
CAS: 77-86-1
Tel: +86-21-56795779
Email: charles7788@worldyachem.com
Mobile: +86-13651600618
QQ: 2850607228
Wechat: 13651600618
WhatsApp: +8613651600618
View History
Raw Materials for Tris(hydroxymethyl) aminomethane
Paraformaldehyde
Paraformaldehyde
Methyl alcohol
Methyl alcohol
tris(hydroxymethyl)nitromethane
Nitromethane
Paraformaldehyde
Methyl alcohol
Methyl alcohol
tris(hydroxymethyl)nitromethane
Nitromethane